Journal: Cancer Research

Article Title: The Androgen Receptor Induces Integrin α6β1 to Promote Prostate Tumor Cell Survival via NF-κB and Bcl-xL Independently of PI3K Signaling

doi: 10.1158/0008-5472.can-10-2745

Figure Lengend Snippet: Figure 5. AR stimulates NF-kB activity. A, B, and F, PC3-Puro (PP), AR1, and AR2 cells, or (C–E) C4-2 and VCaP cells treated with AR or control siRNA, or treated with vehicle (Veh) or R1881. NF-kB activity measured by (A, D–F) immunoblotting for phosphorylated RelA (pRelA) or (B, C) transfection of an NF-kB luciferase reporter. Integrin a6, Bcl-xL, or total RelA measured by immunoblotting. F, control cells not treated (NT) or treated with 10 ng/mL TNFa for 1 hour. G, IKKb (pIKKb) and IkBa (pIkBa) phosphorylation monitored by immunoblotting of immunoprecipitated IKKb or IkBa in total cell lyastes with phospho- specific antibodies; Tub, tubulin.

Article Snippet: Polyclonal antibodies to Bcl-xL, phospho-IkBa S32 (14D4), phospho-NF-kB S536 (93H1), NF-kB p65-RelA, and monoclonal antibodies to IkBa (44D4) were purchased from Cell Signaling.

Techniques: Activity Assay, Control, Western Blot, Transfection, Luciferase, Phospho-proteomics, Immunoprecipitation